Anticorps Polyclonal de lapin anti-MORF4L1

MORF4L1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 55257-1-AP

Synonymes

Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55257-1-AP

  IP Result of anti-MORF4L1 (IP:55257-1-AP, 4ug; Detection:55257-1-AP 1:800) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 55257-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cutané humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55257-1-AP cible MORF4L1 dans les applications de WB, IP, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mortality factor 4 like 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: NM_006791
• Symbole du gène: MORF4L1
• Identification du gène (NCBI): 10933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MORF4L1, also named as MRG15, belongs to the MRG family. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. (PMID:14966270) This antibody has no cross-reaction to MORF4L2.