Anticorps Polyclonal de lapin anti-MMP9 (Middle)

MMP9 (Middle) Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 27306-1-AP

Synonymes

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9, MMP9 (Middle)

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Galerie de données de validation

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  WB analysis of human saliva using 27306-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of human saliva using 27306-1-AP

  IP result of anti-MMP9 (Middle)(IP:27306-1-AP, 4ug; Detection:27306-1-AP 1:300) with human saliva lysate 800 ug.

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  IHC staining of mouse liver using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu salivaire humain,
• Résultats positifs en IP: tissu salivaire humain,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique de souris, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

27306-1-AP cible MMP9 (Middle) dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP9 (Middle) Protéine recombinante Ag26132
• Nom complet: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Masse moléculaire calculée: 707 aa, 78 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC006093
• Symbole du gène: MMP9
• Identification du gène (NCBI): 4318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa or 82 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
human	WB,IHC	Int J Mol Sci; Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion; Authors - Tianxiang Xia
mouse	WB	Front Endocrinol (Lausanne); Nicotinamide mononucleotide attenuates HIF-1α activation and fibrosis in hypoxic adipose tissue via NAD+/SIRT1 axis; Authors - Keke Wu
human	WB	Int J Biol Sci; TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.; Authors - Jiyu Miao
human	WB	Front Mol Biosci; Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer.; Authors - Liang Huang
human	WB	Front Mol Biosci; miR-193a-3p Mediates Placenta Accreta Spectrum Development by Targeting EFNB2 via Epithelial-Mesenchymal Transition Pathway Under Decidua Defect Conditions.; Authors - Na Li
human	WB	Front Mol Biosci; CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.; Authors - Fan Feng