Anticorps Monoclonal anti-MKP-2

MKP-2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1G1C12

N° de cat : 66349-1-Ig

Synonymes

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2

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Galerie de données de validation

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  WB analysis using 66349-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66349-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66349-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66349-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66349-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66349-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66349-1-Ig (MKP-2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66349-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66349-1-Ig(MKP-2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules HepG2, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66349-1-Ig cible MKP-2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MKP-2 Protéine recombinante Ag1206
• Nom complet: dual specificity phosphatase 4
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa, 33 kDa
• Numéro d’acquisition GenBank: BC014565
• Symbole du gène: DUSP4
• Identification du gène (NCBI): 1846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).

Publications

Species	Application	Title
rat	IHC	Mol Neurobiol; Dual Specificity Phosphatases Support Axon Plasticity and Viability.; Authors - Ambika Chandrasekhar
human	WB,IHC	J Cancer; PMID: 31258782; Authors - Xianqing Chen
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu
human,mouse	WB,IHC,CoIP	Cell Rep; DUSP4 promotes esophageal squamous cell carcinoma progression by dephosphorylating HSP90β; Authors - Liting Zhou ; KD Validated
human	IHC	Cancers (Basel); Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases; Authors - Maximiliano Gelli
human	IF	Life Sci Alliance; DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathway; Authors - Yichi Xu