Anticorps Polyclonal de lapin anti-MIP-3 Alpha

MIP-3 Alpha Polyclonal Antibody for IHC,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 26527-1-AP

Synonymes

Beta chemokine exodus 1, C C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, MIP 3 alpha, MIP 3a, MIP3A, MIP-3α, SCYA20, Small inducible cytokine A20, ST38

---

Galerie de données de validation

• 

  IHC staining of human lung cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu d'amygdalite humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

26527-1-AP cible MIP-3 Alpha dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIP-3 Alpha Protéine recombinante Ag24069
• Nom complet: chemokine (C-C motif) ligand 20
• Masse moléculaire calculée: 11 kDa
• Numéro d’acquisition GenBank: BC020698
• Symbole du gène: CCL20
• Identification du gène (NCBI): 6364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

Publications

Species	Application	Title
human	IF	Gut Microbes; Fusobacterium nucleatum promotes colorectal cancer metastasis through miR-1322/CCL20 axis and M2 polarization; Authors - Chaochao Xu
human	IF	Front Cell Dev Biol; EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.; Authors - Bin Qi
mouse		Phytomedicine; The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides.; Authors - Gaofeng Cai
human	IHC	Oncol Rep; DEPDC1 drives hepatocellular carcinoma cell proliferation, invasion and angiogenesis by regulating the CCL20/CCR6 signaling pathway.; Authors - Wubin Guo
human	IHC	Inflammation; PMID: 31838663; Authors - YingYing Hu
human	IHC	Oncol Lett; Overexpression of B7-H4 promotes renal cell carcinoma progression by recruiting tumor-associated neutrophils via upregulation of CXCL8.; Authors - Anqi Li