Anticorps Monoclonal anti-MICA/MICB

MICA/MICB Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

6D4

N° de cat : FITC-65161

Synonymes

MICA, MICB

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Galerie de données de validation

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  FC experiment of HeLa using FITC-65161

  1X10^6 HeLa cells were surface stained with 5 ul FITC Plus Anti-Human MICA/MICB (FITC-65161, Clone:6D4) or Mouse IgG2a Isotype Control. Cells were not fixed. Live cells were gated.

• 

  FC experiment of HeLa using FITC-65161

  1X10^6 HeLa cells were surface stained with 5 ul FITC Plus Anti-Human MICA/MICB (FITC-65161, Clone:6D4) or Mouse IgG2a Isotype Control. Cells were not fixed. Live cells were gated.

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Applications testées

• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

FITC-65161 cible MICA/MICB dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules C1R transfectées exprimant MICA
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 383 aa, 43 kDa
• Numéro d’acquisition GenBank: BC016929
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'isothiocyanate de fluorescéine (FITC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Publications

Species	Application	Title
	FC	Biosci Microbiota Food Health; Diverse impact of a probiotic strain, Lacticaseibacillus paracasei Shirota, on peripheral mononuclear phagocytic cells in healthy Japanese office workers: a randomized, double-blind, controlled trial; Authors - Tomoaki Naito