Anticorps Monoclonal anti-MICA/MICB

MICA/MICB Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

6D4

N° de cat : CL750-65161

Synonymes

FLJ60820, MIC A, MICA, PERB11.1

---

Galerie de données de validation

• 

  FC experiment of HeLa using CL750-65161

  1X10^6 HeLa cells were surface stained with 5 ul CoraLite® Plus 750 Anti-Human MICA/MICB (CL750-65161, Clone:6D4). Cells were not fixed.

• 

  FC experiment of HeLa using CL750-65161

  1X10^6 HeLa cells were surface stained with 5 ul CoraLite® Plus 750 Anti-Human MICA/MICB (CL750-65161, Clone:6D4). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65161 cible MICA/MICB dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules C1R transfectées exprimant MICA
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 383 aa, 43 kDa
• Numéro d’acquisition GenBank: BC016929
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.