Anticorps Monoclonal anti-MICA/MICB

MICA/MICB Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

WB, FC

Conjugaison

Non conjugué

Publications

3

CloneNo.

6D4

N° de cat : 65161-1-Ig

Synonymes

MICA, MICB

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Galerie de données de validation

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  FC experiment of HeLa using 65161-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB (65161-1-Ig, Clone: 6D4) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

• 

  FC experiment of HeLa using 65161-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB (65161-1-Ig, Clone: 6D4) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

65161-1-Ig cible MICA/MICB dans les applications de WB, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules C1R transfectées exprimant MICA
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 383 aa, 43 kDa
• Numéro d’acquisition GenBank: BC016929
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Publications

Species	Application	Title
human	WB	J Immunother; BCMA-targeting Bispecific Antibody That Simultaneously Stimulates NKG2D-enhanced Efficacy Against Multiple Myeloma.; Authors - Yang Wang
human	WB	Int Immunopharmacol; Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway.; Authors - Hui-Yang Wu
human	WB	Cancers (Basel); circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis; Authors - Fei Liu