Anticorps Monoclonal anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications

2

CloneNo.

M5/114.15.2

N° de cat : CL647-65122

Synonymes

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65122

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) at dilution of 1:400.

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  FC experiment of BALB/c mouse splenocytes using CL647-65122

  1X10^6 BALB/c mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.25 ug CoraLite® Plus 647 Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris BALB/c,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

CL647-65122 cible MHC Class II (I-A/I-E) dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules spléniques activées de souris C57BL/6
• Nom complet: histocompatibility 2, class II antigen A, alpha
• Numéro d’acquisition GenBank: BC031711
• Symbole du gène: H2-Aa
• Identification du gène (NCBI): 14960
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Publications

Species	Application	Title
mouse	FC	Vet Res; Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis; Authors - Luqiu Feng
	IF	Pharmaceuticals (Basel); Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells; Authors - Natalia Sauer