Anticorps Monoclonal anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

Non conjugué

Publications

1

CloneNo.

M5/114.15.2

N° de cat : 65122-1-Ig

Synonymes

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 65122-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (65122-1-Ig, Clone: M5/114.15.2) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

65122-1-Ig cible MHC Class II (I-A/I-E) dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules spléniques activées de souris C57BL/6
• Nom complet: histocompatibility 2, class II antigen A, alpha
• Numéro d’acquisition GenBank: BC031711
• Symbole du gène: H2-Aa
• Identification du gène (NCBI): 14960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Publications

Species	Application	Title
mouse	IF	Int Immunopharmacol; Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4+ T cells in chronic liver injury; Authors - Yuwei Zhang