Anticorps Monoclonal anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

M5/114.15.2

N° de cat : CL488-65122

Synonymes

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL488-65122

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse MHC Class II (I-A/I-E) (CL488-65122, Clone: M5/114.15.2) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL488-65122

  1X10^6 mouse splenocytes were surface co-stained with CoraLite®647 Anti-Mouse CD3 (17A2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (CL488-65122, Clone:M5/114.15.2) or 0.25 ug CoraLite® Plus 488-conjugated Rat IgG2b isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65122 cible MHC Class II (I-A/I-E) dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules spléniques activées de souris C57BL/6
• Nom complet: histocompatibility 2, class II antigen A, alpha
• Numéro d’acquisition GenBank: BC031711
• Symbole du gène: H2-Aa
• Identification du gène (NCBI): 14960
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).