Anticorps Monoclonal anti-MGP

MGP Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1A1C3

N° de cat : 60055-1-Ig

Synonymes

GIG36, matrix Gla protein, MGLAP, MGP, NTI

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Galerie de données de validation

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  WB analysis of human placenta using 60055-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 60055-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver cancer using 60055-1-Ig

  human liver cancer tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60055-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 60055-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human heart using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human heart using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human kidney using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human kidney using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human lung using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDA-MB-231 using 60055-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 60055-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, salive humain, salive humaine, tissu cérébral humain, tissu de cancer du foie humain, tissu rénal humain
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain, tissu cérébral humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDA-MB-231, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

60055-1-Ig cible MGP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MGP Protéine recombinante Ag1091
• Nom complet: matrix Gla protein
• Masse moléculaire calculée: 103 aa, 13 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC005272
• Symbole du gène: MGP
• Identification du gène (NCBI): 4256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Publications

Species	Application	Title
human	WB	J Inflamm Res; circ_TGFBR2 Inhibits Vascular Smooth Muscle Cells Phenotypic Switch and Suppresses Aortic Dissection Progression by Sponging miR-29a; Authors - Zhenjun Xu
human	WB	J Biol Chem; Reduced APPL1 impairs osteogenic differentiation of mesenchymal stem cells by facilitating MGP expression to disrupt the BMP2 pathway in osteoporosis; Authors - Weiquan Yuan ; KD Validated