Anticorps Monoclonal anti-MGEA5

MGEA5 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3B9H7

N° de cat : 66033-1-Ig

Synonymes

Beta N acetylhexosaminidase, Bifunctional protein NCOAT, HAT, HEXC, Hexosaminidase C, KIAA0679, MEA5, Meningioma expressed antigen 5, MGEA5, N acetyl beta glucosaminidase, NCOAT, O GlcNAcase, OGA

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Galerie de données de validation

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  WB analysis of Human brain using 66033-1-Ig

  Human brain lysate were subjected to SDS PAGE followed by western blot with 66033-1-Ig (MGEA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 66033-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66033-1-Ig (MGEA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human meningioma using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human meningioma using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human brain using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 66033-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66033-1-Ig (MGEA5 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HeLa using 66033-1-Ig

  Immunofluorescent analysis of Hela cells, using MGEA5 antibody 66033-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules MCF7
• Résultats positifs en IHC: tissu de méningiome humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66033-1-Ig cible MGEA5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MGEA5 Protéine recombinante Ag6905
• Nom complet: meningioma expressed antigen 5 (hyaluronidase)
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 75 kDa, 130 kDa
• Numéro d’acquisition GenBank: BC039583
• Symbole du gène: MGEA5
• Identification du gène (NCBI): 10724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present mouse monopolyclonal antibody 66033-1-Ig, which is consistent with results in a related reference (PubMed:11341771).

Publications

Species	Application	Title
mouse	WB	Res Sq; Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction; Authors - Hossein Ardehali