Anticorps Monoclonal anti-METTL7A

• Phare
• Validé par KD/KO

METTL7A Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A9B4

N° de cat : 67905-1-Ig

Synonymes

AAM B, DKFZp586A0522, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

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Galerie de données de validation

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  WB analysis using 67905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67905-1-Ig

  WB result of METTL7A antibody (67905-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

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  WB analysis of mouse lung using 67905-1-Ig

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67905-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules HepG2, cellules K-562, cellules LNCaP, tissu hépatique de lapin, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67905-1-Ig cible METTL7A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: METTL7A Protéine recombinante Ag29986
• Nom complet: methyltransferase like 7A
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC008180
• Symbole du gène: METTL7A
• Identification du gène (NCBI): 25840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.