Anticorps Monoclonal anti-METTL3

• Phare
• Validé par KD/KO

METTL3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

2D8H1

N° de cat : 67733-1-Ig

Synonymes

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

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Galerie de données de validation

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  WB analysis using 67733-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67733-1-Ig (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of A549 using 67733-1-Ig

  WB result of METTL3 antibody (67733-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected A549 cells.

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  WB analysis of A549 using 67733-1-Ig

  WB result of METTL3 antibody (67733-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected A549 cells.

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  IHC staining of human lung cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 67733-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using METTL3 antibody (67733-1-Ig, Clone: 2D8H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse testis using 67733-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using METTL3 antibody (67733-1-Ig, Clone: 2D8H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293 using 67733-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human METTL3 (67733-1-Ig, Clone:2D8H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'œsophage humain, tissu de cancer du côlon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu testiculaire de souris,
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Informations sur le produit

67733-1-Ig cible METTL3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: METTL3 Protéine recombinante Ag7110
• Nom complet: methyltransferase like 3
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC001650
• Symbole du gène: METTL3
• Identification du gène (NCBI): 56339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Publications

Species	Application	Title
human	IP	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
human	WB,IF,IP	J Invest Dermatol; NPTX2 Promotes EMT in Cutaneous Squamous Cell Carcinoma via Mettl3-Mediated m6A Methylation of Snail; Authors - Tong Kang
human,mouse	WB,IF	Am J Respir Cell Mol Biol; The Combined Effects of Circular RNA Methylation Promote Pulmonary Fibrosis.; Authors - Sha Wang ; KD Validated
human,mouse	IHC	Evid Based Complement Alternat Med; m6A RNA Methylation Regulators Contribute to Predict and as a Therapy Target of Pulmonary Fibrosis.; Authors - Meng-Sheng Deng
mouse	WB,IHC	Int J Mol Sci; Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model; Authors - Yanan Wang ; KO Validated
human	WB	Apoptosis; NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer; Authors - Yang Wang