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Anticorps Monoclonal anti-MEK1

MEK1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F6D7

N° de cat : CL488-67872

Synonymes

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, PRKMK1

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated MEK1 Monoclonal anti (CL488-67872)

    IF Staining of A431 using CL488-67872

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MEK1 antibody (CL488-67872, Clone: 3F6D7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated MEK1 Monoclonal anti (CL488-67872)

    FC experiment of A431 using CL488-67872

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MEK1 (CL488-67872, Clone:3F6D7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules A431,
Résultats positifs en cytométriecellules A431

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67872 cible MEK1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet mitogen-activated protein kinase kinase 1
Masse moléculaire calculée 43 kDa
Poids moléculaire observé40-50 kDa
Numéro d’acquisition GenBankBC139729
Symbole du gène MAP2K1
Identification du gène (NCBI) 5604
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 MEK1 antibody CL488-67872Download protocol
FC protocol for CL Plus 488 MEK1 antibody CL488-67872Download protocol
Standard Protocols
Click here to view our Standard Protocols