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Anticorps Polyclonal de lapin anti-MED23

MED23 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 27850-1-AP

Synonymes

ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2

Galerie de données de validation

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  • Western Blot (WB) analysis of A375 cells using MED23 Polyclonal antibody (27850-1-AP)

    WB analysis of A375 using 27850-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27850-1-AP (MED23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules A375,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

27850-1-AP cible MED23 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéesouris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MED23 Protéine recombinante Ag27241
Nom complet mediator complex subunit 23
Masse moléculaire calculée 156 kDa
Poids moléculaire observé 140 kDa, 50 kDa
Numéro d’acquisition GenBankBC060759
Symbole du gène MED23
Identification du gène (NCBI) 9439
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

Protocole

Product Specific Protocols
WB protocol for MED23 antibody 27850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Authors - Xu Wang
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

SITING (Verified Customer) (04-02-2021)

not very good， too many unspecific band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED23 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:27850-1-AP)