Anticorps Polyclonal de lapin anti-MECP2

• Phare
• Validé par KD/KO

MECP2 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-10861

Synonymes

AUTSX3, DKFZp686A24160, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-10861

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MECP2 antibody (CL488-10861) at dilution of 1:100, CL594-Phalloidin (red).

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  FC experiment of NIH/3T3 using CL488-10861

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human MECP2 (CL488-10861) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-10861 cible MECP2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MECP2 Protéine recombinante Ag1309
• Nom complet: methyl CpG binding protein 2 (Rett syndrome)
• Masse moléculaire calculée: 52 kDa, 53 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC011612
• Symbole du gène: MECP2
• Identification du gène (NCBI): 4204
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011