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Anticorps Monoclonal anti-MCL1

MCL1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3C2

N° de cat : CL488-66026

Synonymes

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated MCL1 Monoclonal anti (CL488-66026)

    IF Staining of HeLa using CL488-66026

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MCL1 antibody (CL488-66026, Clone: 1E3C2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MCL1 Monoclonal anti (CL488-66026)

    FC experiment of HeLa using CL488-66026

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MCL1 (CL488-66026, Clone:1E3C2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66026 cible MCL1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène MCL1 Protéine recombinante Ag10609
Nom complet myeloid cell leukemia sequence 1 (BCL2-related)
Masse moléculaire calculée 350 aa, 37 kDa
Numéro d’acquisition GenBankBC107735
Symbole du gène MCL1
Identification du gène (NCBI) 4170
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 MCL1 antibody CL488-66026Download protocol
Standard Protocols
Click here to view our Standard Protocols