Anticorps Monoclonal anti-MBIP

• Phare
• Validé par KD/KO

MBIP Monoclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IP, IHC,ELISA

Conjugaison

Non conjugué

CloneNo.

2E6H11

N° de cat : 66102-1-Ig

Synonymes

MBIP, MUK binding inhibitory protein

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Galerie de données de validation

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  WB analysis using 66102-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66102-1-Ig

  WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

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  WB analysis of human heart using 66102-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66102-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66102-1-Ig

  IP Result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug.

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  IHC staining of human kidney using 66102-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66102-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66102-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66102-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules THP-1, tissu cardiaque humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66102-1-Ig cible MBIP dans les applications de WB, IP, IHC,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MBIP Protéine recombinante Ag18090
• Nom complet: MAP3K12 binding inhibitory protein 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC005197
• Symbole du gène: MBIP
• Identification du gène (NCBI): 51562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.