Anticorps Polyclonal de lapin anti-JNK

JNK Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 51153-1-AP

Synonymes

c Jun N terminal kinase 2, JNK 55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAP kinase 9, MAPK 9, MAPK9, p54a, p54aSAPK, PRKM9, SAPK

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Galerie de données de validation

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  WB analysis of HeLa using 51153-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 51153-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 51153-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 51153-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 51153-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 51153-1-AP

  IP Result of anti-JNK (IP:51153-1-AP, 4ug; Detection:51153-1-AP 1:1000) with HeLa cells lysate 4000ug.

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  IF Staining of HeLa using 51153-1-AP

  Immunofluorescent analysis of HeLa cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HUVEC using 51153-1-AP

  Immunofluorescent analysis of HUVEC cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules COS-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HeLa, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

51153-1-AP cible JNK dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JNK Protéine recombinante Ag1442
• Nom complet: mitogen-activated protein kinase 9
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 44-55 kDa
• Numéro d’acquisition GenBank: BC032539
• Symbole du gène: MAPK9
• Identification du gène (NCBI): 5601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publications

Species	Application	Title
human	WB	Adv Mater; m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation; Authors - Qing You
human	WB	Nat Commun; SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.; Authors - Peiyuan Zhang
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun
rat	WB	Neurochem Res; Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.; Authors - Xuehua Sun
rabbit	WB	Microb Pathog; Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis.; Authors - Liyan Chen
mouse	IHC	J Ethnopharmacol; Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro; Authors - Xiao-Li Li