Anticorps Polyclonal de lapin anti-MAP2

MAP2 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Avis (1)

N° de cat : CL594-17490

Synonymes

DKFZp686I2148, MAP 2, MAP2, MAP2A, MAP2B, MAP2C

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Galerie de données de validation

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  IF Staining of rat brain using CL594-17490

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 MAP2 antibody (CL594-17490) at dilution of 1:200, CoraLite®488 GFAP antibody (CL488-16825, green). DAPI (blue).

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  IF Staining of rat brain using CL594-17490

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 MAP2 antibody (CL594-17490) at dilution of 1:200, CoraLite®488 GFAP antibody (CL488-16825, green). DAPI (blue).

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  IF Staining of rat brain using CL594-17490

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 MAP2 antibody (CL594-17490) at dilution of 1:200. DAPI (blue).

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  FC experiment of Neuro-2a using CL594-17490

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2 (CL594-17490) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu cérébral de rat,
• Résultats positifs en cytométrie: cellules Neuro-2a

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-17490 cible MAP2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP2 Protéine recombinante Ag11580
• Nom complet: microtubule-associated protein 2
• Masse moléculaire calculée: 200 kDa
• Numéro d’acquisition GenBank: BC038857
• Symbole du gène: MAP2
• Identification du gène (NCBI): 4133
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.

What is the molecular weight of MAP2? Is MAP2 post-translationally modified?

MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).

What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?

MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.

Can MAP2 be used as a neuronal marker?

MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Annette (Verified Customer) (05-13-2022)

It is possible to reduce the concentration to 1:500 or even 1:1000 and get a good signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Cerebral cortex of rat

CL594 MAP2 Antibody Immunofluorescence validation (1:250 dilution) in Cerebral cortex of rat (Cat no:CL594-17490)