Anticorps Monoclonal anti-MAP1B

MAP1B Monoclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E6B9

N° de cat : 67423-1-Ig

Synonymes

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5

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Galerie de données de validation

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  IHC staining of rat brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 67423-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67423-1-Ig (MAP1B antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of SH-SY5Y using 67423-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human MAP1B (67423-1-Ig, Clone:1E6B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat,
• Résultats positifs en cytométrie: cellules SH-SY5Y,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67423-1-Ig cible MAP1B dans les applications de IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAP1B Protéine recombinante Ag17709
• Nom complet: microtubule-associated protein 1B
• Masse moléculaire calculée: 2468 aa, 271 kDa
• Numéro d’acquisition GenBank: BC141853
• Symbole du gène: MAP1B
• Identification du gène (NCBI): 4131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)