Anticorps Monoclonal anti-Ly-6G/Ly-6C (Gr-1)

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

Publications

2

CloneNo.

RB6-8C5

N° de cat : 65140-1-Ig

Synonymes

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using 65140-1-Ig

  1X10^6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD11b and 0.5 ug Anti-Mouse Ly-6G/Ly-6C (Gr-1) (65140-1-Ig, Clone:RB6-8C5) and FITC anti-Rat IgG2b Antibody at dilution 1:1000, or 0.5 ug Rat IgG2b isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

65140-1-Ig cible Ly-6G/Ly-6C (Gr-1) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Produit contre des granulocytes d'origine murine
• Nom complet: lymphocyte antigen 6 complex, locus G
• Numéro d’acquisition GenBank: X70920
• Symbole du gène: Ly6g
• Identification du gène (NCBI): 546644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publications

Species	Application	Title
mouse	FC	J Leukoc Biol; The effect of an isoquinoline alkaloid on treatment of periodontitis by regulating the neutrophils chemotaxis.; Authors - Yitong Liu
mouse	FC	J Oncol; Blocking TGF-β Expression Attenuates Tumor Growth in Lung Cancers, Potentially Mediated by Skewing Development of Neutrophils.; Authors - Yifei Fang