Anticorps Monoclonal anti-Lamin B1

Lamin B1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

2

CloneNo.

3C10G12

N° de cat : CL594-66095

Synonymes

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-66095

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Lamin B1 antibody (CL594-66095, Clone: 3C10G12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66095

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Lamin B1 (CL594-66095, Clone:3C10G12) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL594-66095 cible Lamin B1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lamin B1 Protéine recombinante Ag20522
• Nom complet: lamin B1
• Masse moléculaire calculée: 66 kDa
• Numéro d’acquisition GenBank: BC012295
• Symbole du gène: LMNB1
• Identification du gène (NCBI): 4001
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
human	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
human	IF	Mol Oncol; Small extracellular vesicles deliver TGF-β1 and promote adriamycin resistance in breast cancer cells.; Authors - Chunli Tan

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011