Anticorps Recombinant de lapin anti-Lamin B1

• Phare
• Validé par KD/KO

Lamin B1 Recombinant Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

5H10

N° de cat : 80906-1-RR

Synonymes

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

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Galerie de données de validation

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  WB analysis using 80906-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80906-1-RR (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 80906-1-RR

  WB result of Lamin B1 antibody (80906-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells.

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  IHC staining of human colon cancer using 80906-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 80906-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 80906-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 80906-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

80906-1-RR cible Lamin B1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lamin B1 Protéine recombinante Ag3631
• Nom complet: lamin B1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC012295
• Symbole du gène: LMNB1
• Identification du gène (NCBI): 4001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Publications

Species	Application	Title
human	WB	ACS Nano; Targeted Degradation of Signal Transduction and Activator of Transcription 3 by Chaperone-Mediated Autophagy Targeting Chimeric Nanoplatform; Authors - Haohao Song