Anticorps Monoclonal anti-LYN

LYN Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3C7F2

N° de cat : 60211-1-Ig

Synonymes

JTK8, LYN, Tyrosine protein kinase Lyn

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 60211-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60211-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60211-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 60211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 60211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

60211-1-Ig cible LYN dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LYN Protéine recombinante Ag12723
• Nom complet: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Masse moléculaire calculée: 512 aa, 59 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC075002
• Symbole du gène: LYN
• Identification du gène (NCBI): 4067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
	WB	Cancer Res; Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells; Authors - Li Xia
mouse	WB	Food Funct; Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation; Authors - Wei Xu
human	IHC	Life Sci; Integrated proteomics and phosphoproteomics analyses of esophageal cancer cells with different invasive abilities; Authors - Nansong Xu