Anticorps Monoclonal anti-LRPPRC

• Phare
• Validé par KD/KO

LRPPRC Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3H7E1

N° de cat : 67679-1-Ig

Synonymes

130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC

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Galerie de données de validation

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  WB analysis using 67679-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67679-1-Ig

  WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells.

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  WB analysis of U-251 using 67679-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HEK-293 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue).

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  IF Staining of HepG2 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HeLa, cellules HepG2, cellules U-251
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'endomètre humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules A431, cellules HEK-293, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67679-1-Ig cible LRPPRC dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LRPPRC Protéine recombinante Ag30261
• Nom complet: leucine-rich PPR-motif containing
• Masse moléculaire calculée: 1394 aa, 158 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC050311
• Symbole du gène: LRPPRC
• Identification du gène (NCBI): 10128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Publications

Species	Application	Title
human	WB	Front Genet; RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke; Authors - Kejuan Jia