Anticorps Polyclonal de lapin anti-LIG3

LIG3 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 26583-1-AP

Synonymes

DNA ligase 3, DNA ligase III, LIG2, LIG3, ligase III, DNA, ATP dependent

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Galerie de données de validation

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  WB analysis of HEK-293T using 26583-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP (LIG3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26583-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP (LIG3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 26583-1-AP

  IP Result of anti-LIG3 (IP:26583-1-AP, 4ug; Detection:26583-1-AP 1:300) with HEK-293T cells lysate 4000ug.

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  IF Staining of HeLa using 26583-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26583-1-AP (LIG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T
• Résultats positifs en IP: cellules HEK-293T
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

26583-1-AP cible LIG3 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIG3 Protéine recombinante Ag24998
• Nom complet: ligase III, DNA, ATP-dependent
• Masse moléculaire calculée: 100-110 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC068005
• Symbole du gène: LIG3
• Identification du gène (NCBI): 3980
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA ligase 3 is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms

Publications

Species	Application	Title
human	IF	Brain; Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy.; Authors - Elena Bonora
pig	WB	Int J Biochem Cell Biol; Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.; Authors - Guoling Li
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
human	WB	Nature; Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesis; Authors - Fu Yang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T