Anticorps Polyclonal de lapin anti-Galectin-4

Galectin-4 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27552-1-AP

Synonymes

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

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Galerie de données de validation

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  WB analysis using 27552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27552-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 27552-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 27552-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 27552-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Galectin-4 antibody (27552-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu de côlon de rat, tissu de côlon de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

27552-1-AP cible Galectin-4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Galectin-4 Protéine recombinante Ag26130
• Nom complet: lectin, galactoside-binding, soluble, 4
• Masse moléculaire calculée: 323 aa, 36 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC034750
• Symbole du gène: LGALS4
• Identification du gène (NCBI): 3960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone.; Authors - Yanlu Wang
human	WB	Cell Adh Migr; SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.; Authors - Yusuke Mizutani