Anticorps Polyclonal de lapin anti-LC3

LC3 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-14600

Synonymes

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3

---

Galerie de données de validation

• 

  IF Staining of HepG2 using CL488-14600

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 LC3 antibody (CL488-14600) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-14600

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 LC3 antibody (CL488-14600) at dilution of 1:200.

• 

  FC experiment of HeLa using CL488-14600

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LC3 (CL488-14600) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HEK-293 using CL488-14600

  1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LC3 (CL488-14600) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF: cellules HepG2 traitées à la chloroquine,
• Résultats positifs en cytométrie: cellules HeLa, cellules HEK-293

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-14600 cible LC3 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LC3 Protéine recombinante Ag6144
• Nom complet: microtubule-associated protein 1 light chain 3 beta
• Masse moléculaire calculée: 15 kDa
• Numéro d’acquisition GenBank: BC067797
• Symbole du gène: MAP1LC3B
• Identification du gène (NCBI): 81631
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C