Anticorps Monoclonal anti-LATS1

• Phare
• Validé par KD/KO

LATS1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2A8F1

N° de cat : 66569-1-Ig

Synonymes

h warts, LATS1, WARTS, WARTS protein kinase, wts

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Galerie de données de validation

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  WB analysis using 66569-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66569-1-Ig

  WB result of LATS1 antibody (66569-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells.

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  WB analysis of HepG2 using 66569-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66569-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66569-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66569-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66569-1-Ig cible LATS1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LATS1 Protéine recombinante Ag11140
• Nom complet: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Masse moléculaire calculée: 15 kDa, 127 kDa
• Poids moléculaire observé: 140-150 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC015665
• Symbole du gène: LATS1
• Identification du gène (NCBI): 9113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongds to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).