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Anticorps Monoclonal anti-L1CAM

L1CAM Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3H7B9

N° de cat : CL594-67115

Synonymes

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated L1CAM Monoclonal antibody (CL594-67115)

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated L1CAM Monoclonal antibody (CL594-67115)

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

Applications testées

Résultats positifs en IFtissu cérébral de souris,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67115 cible L1CAM dans les applications de IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène L1CAM Protéine recombinante Ag17706
Nom complet L1 cell adhesion molecule
Masse moléculaire calculée 1257 aa, 140 kDa
Poids moléculaire observé 220 kDa
Numéro d’acquisition GenBankBC126229
Symbole du gène L1CAM
Identification du gène (NCBI) 3897
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

Protocole

Product Specific Protocols
IF protocol for CL594 L1CAM antibody CL594-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols