Anticorps Recombinant de lapin anti-Cytokeratin 20

Cytokeratin 20 Recombinant Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H10

N° de cat : 82428-1-RR

Synonymes

CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

---

Galerie de données de validation

• 

  WB analysis using 82428-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 82428-1-RR

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 82428-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 82428-1-RR

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules COLO 320, tissu de côlon de porc, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de cancer du côlon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HT-29,
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82428-1-RR cible Cytokeratin 20 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 20 Protéine recombinante Ag11308
• Nom complet: keratin 20
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC031559
• Symbole du gène: KRT20
• Identification du gène (NCBI): 54474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.