Anticorps Recombinant de lapin anti-Cytokeratin 18

Cytokeratin 18 Recombinant Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

4F2

N° de cat : 82817-5-RR

Synonymes

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie de données de validation

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  WB analysis using 82817-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HCT 116, cellules HepG2, cellules K-562, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82817-5-RR cible Cytokeratin 18 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 18 Protéine recombinante Ag1260
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: KRT18
• Identification du gène (NCBI): 3875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.