Anticorps Monoclonal anti-Cytokeratin 14

Cytokeratin 14 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

CloneNo.

2G1E2

N° de cat : 60320-1-Ig

Synonymes

Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Galerie de données de validation

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  WB analysis of A431 using 60320-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 60320-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 1010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 60320-1-Ig

  Immunofluorescent analysis of A431 cells using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

• 

  IF Staining of mouse skin using 60320-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 14 antibody (60320-1-Ig, Clone: 2G1E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skin using 60320-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 14 antibody (60320-1-Ig, Clone: 2G1E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cutané de rat, tissu cutané humain, tissu de cancer de la peau humain, tissu de cancer du col de l'utérus humain, tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, tissu cutané de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:800
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Informations sur le produit

60320-1-Ig cible Cytokeratin 14 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 14 Protéine recombinante Ag17559
• Nom complet: keratin 14
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC002690
• Symbole du gène: KRT14
• Identification du gène (NCBI): 3861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Publications

Species	Application	Title
mouse	IF	Small; Eco-Friendly and Scalable Synthesis of Fullerenols with High Free Radical Scavenging Ability for Skin Radioprotection.; Authors - Maoru Zhao
human	WB	Breast Cancer Res; Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.; Authors - Dongdong Wang
mouse	WB,IHC	Environ Sci Technol; Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2.; Authors - Tingting Ku
mouse	IF	Stem Cell Reports; SOX2 Is a Marker for Stem-like Tumor Cells in Bladder Cancer.; Authors - Fengyu Zhu
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	IF	Stem Cell Res Ther; Bone marrow mesenchymal stem cells facilitate diabetic wound healing through the restoration of epidermal cell autophagy via the HIF-1α/TGF-β1/SMAD pathway; Authors - Yan Shi