Anticorps Polyclonal de lapin anti-KMT2D

• Phare
• Validé par KD/KO

KMT2D Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 27266-1-AP

Synonymes

AAD10, ALL1 related protein, ALR, CAGL114, KMT2D, Lysine N methyltransferase 2B, MLL2, MLL4, TNRC21

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Galerie de données de validation

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  WB analysis using 27266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27266-1-AP (KMT2D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• ChIP: See 2 publications below

Informations sur le produit

27266-1-AP cible KMT2D dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KMT2D Protéine recombinante Ag26195
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia 2
• Masse moléculaire calculée: 593 kDa
• Poids moléculaire observé: 593 kDa
• Numéro d’acquisition GenBank: NM_003482
• Symbole du gène: MLL2
• Identification du gène (NCBI): 8085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KMT2D (a COMPASS/ Set1 family member; also known as MLL4, ALR, and MLL2) is the biggest H3K4 methyltransferase among the most frequently mutated genes in many different types of cancer (PMID: 34194626). The enzymatic function of KMT2D depends on a cluster of C-terminal conserved domains, including a PHD domain, two FY-rich motifs (FYRC and FYRN) and a catalytic SET domain. KMT2D has two frequently occurring genomic alterations: truncation and missense mutations. Loss of KMT2D is a bona fide tumor suppressor gene in FL and DLBCL. Kmt2d perturbed the expression of genes that sustain proliferation and survival, and the KMT2D protein directly binds and associates with an active chromatin conformation in negative modulators of the BCR and lymphocyte migration pathways, which in turn could affect B cell responses to antigen(PMID: 26366712).

Publications

Species	Application	Title
human	WB	Cancer Immunol Res; Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis.; Authors - Xuanwen Bao
human	IHC	Oncogene; Histone 3 lysine-27 demethylase KDM6A coordinates with KMT2B to play an oncogenic role in NSCLC by regulating H3K4me3.; Authors - Xuejiao Leng
human	IHC,IF	Cell Biosci; Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma.; Authors - Xinmiao Wang
human	IHC	Thorac Cancer; RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.; Authors - Ning Jiang
	ChIP	Cell Death Discov; Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ1-42 microenvironment.; Authors - Qianshuo Liu
human	WB,IHC,IF	World J Gastrointest Oncol; KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.; Authors - Si-Si Li ; KD Validated