Anticorps Polyclonal de lapin anti-KHSRP

• Phare
• Validé par KD/KO

KHSRP Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, RIP, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 55409-1-AP

Synonymes

FBP2, FUBP2, FUSE binding protein 2, KHSRP, KSRP, p75

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Galerie de données de validation

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  WB analysis using 55409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55409-1-AP (KHSRP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55409-1-AP

  WB result of KHSRP antibody (55409-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHSRP transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

55409-1-AP cible KHSRP dans les applications de WB, RIP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: KH-type splicing regulatory protein
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_003685
• Symbole du gène: KHSRP
• Identification du gène (NCBI): 8570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
human	WB,IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Atherosclerosis; microRNA-185 modulates low density lipoprotein receptor expression as a key posttranscriptional regulator.; Authors - Huajun Jiang
human	WB	Int Immunopharmacol; Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.; Authors - Kaiyong Yang ; KD Validated
human	WB,RIP	Cell Rep; Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.; Authors - Zhenzhen Chen ; KO Validated
human	WB, IF	Biochim Biophys Acta; Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.; Authors - Fan Chai