Anticorps Monoclonal anti-KGA-Specific

KGA-Specific Monoclonal Antibody for IF, WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11A11

N° de cat : CL488-66265

Synonymes

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase

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Galerie de données de validation

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  WB analysis using CL488-66265

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66265 (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cérébral de souris
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66265 cible KGA-Specific dans les applications de WB, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KGA-Specific Protéine recombinante Ag24227
• Nom complet: glutaminase
• Masse moléculaire calculée: 669 aa, 73 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC038507
• Symbole du gène: GLS
• Identification du gène (NCBI): 2744
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.