Anticorps Monoclonal anti-KDM1

KDM1 Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2F10A12

Avis (1)

N° de cat : 67037-1-Ig

Synonymes

AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1

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Galerie de données de validation

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  WB analysis using 67037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67037-1-Ig cible KDM1 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KDM1 Protéine recombinante Ag14778
• Nom complet: lysine (K)-specific demethylase 1
• Masse moléculaire calculée: 876 aa, 95 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC040194
• Symbole du gène: KDM1
• Identification du gène (NCBI): 23028
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.

Publications

Species	Application	Title
human	WB	Life Sci; MicroRNA-325 facilitates atherosclerosis progression by mediating the SREBF1/LXR axis via KDM1A.; Authors - Yanhua Pu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (10-21-2020)

Observed band at the right size with a clean background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver lysate

KDM1 Antibody Western Blot validation (1:2000 dilution) in Mouse liver lysate (Cat no:67037-1-Ig)