Anticorps Polyclonal de lapin anti-KCTD10

• Phare
• Validé par KD/KO

KCTD10 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 27279-1-AP

Synonymes

KCTD10, MSTP028, ULR061, ULRO61

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Galerie de données de validation

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  WB analysis of Jurkat using 27279-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 27279-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27279-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27279-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27279-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27279-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27279-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules K-562, cellules MCF-7, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below

Informations sur le produit

27279-1-AP cible KCTD10 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCTD10 Protéine recombinante Ag25971
• Nom complet: potassium channel tetramerisation domain containing 10
• Masse moléculaire calculée: 313 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC040062
• Symbole du gène: KCTD10
• Identification du gène (NCBI): 83892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Unbiased interrogation of functional lysine residues in human proteome; Authors - Ying Bao
human	WB	Nat Commun; Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.; Authors - Xin Wu ; KO Validated
mouse	WB	Front Cell Dev Biol; Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.; Authors - Yue Liu ; KD Validated
human,rat	WB	Genet Res (Camb); Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis; Authors - Yun Feng ; KD Validated