Anticorps Monoclonal anti-KChIP1

KChIP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3D6C1

N° de cat : 66439-1-Ig

Synonymes

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

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Galerie de données de validation

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  WB analysis of human cerebellum using 66439-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66439-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66439-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 66439-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66439-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66439-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66439-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 66439-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 66439-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de cervelet humain, tissu cérébral humain fœtal, tissu splénique humain
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66439-1-Ig cible KChIP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KChIP1 Protéine recombinante Ag5494
• Nom complet: Kv channel interacting protein 1
• Masse moléculaire calculée: 227 aa, 27 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC050375
• Symbole du gène: KCNIP1
• Identification du gène (NCBI): 30820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.