Anticorps Polyclonal de lapin anti-KCC4/SLC12A7

KCC4/SLC12A7 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 26590-1-AP

Synonymes

K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7

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Galerie de données de validation

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  WB analysis using 26590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26590-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules NCI-H1299, cellules Raji, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26590-1-AP cible KCC4/SLC12A7 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCC4/SLC12A7 Protéine recombinante Ag24954
• Nom complet: solute carrier family 12 (potassium/chloride transporters), member 7
• Poids moléculaire observé: 119 kDa
• Numéro d’acquisition GenBank: BC098390
• Symbole du gène: SLC12A7
• Identification du gène (NCBI): 10723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCC4, also named as Solute carrier family 12 member 7(SLC12A7), is a 1083 amino-acid protein, which belongs to the SLC12A transporter family. As a multi-pass membrane protein, the molecular weight of KCC4 is 119 kDa. KCC4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. Moreover, KCC4 may mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti(Uniprot, GeneID:10723).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bishwa Ranjan (Verified Customer) (08-03-2023)

In the Western blots there is an additional band below at 98kD which seems like a degradation or non-specific binding of the antibody. The bands showing at 120-130kD are accurate as we have seen in the lanes from knockdowns and overexpression of protein KCC4 For reference information about the lanes: 1. MDA KCC4 overexpression 2. MDA KCC4 knockdown sequence1 3. MDA KCC4 knockdown sequence2 4. MDA KCC4 knockdown sequence3 5. MDA scrambled control As you can see in lane 5 which is the control has expression of kCC4 at 120-130 range and for the knockdowns there is a decrease more significantly for 2nd sequence and for overexpression in lane 1 it is quite high so we believe the top band is the expected one and the bottom one is a result of degradation maybe so please advice on this matter. As for the antibody we received it in April 2023 and it is stored at -20 as mentioned.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MDA-MB-231, A-431

KCC4/SLC12A7 Antibody Western Blot validation (1:2000 dilution) in MDA-MB-231, A-431 (Cat no:26590-1-AP)