Anticorps Monoclonal anti-KAP1

• Phare
• Validé par KD/KO

KAP1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

1B9G12

N° de cat : 66630-1-Ig

Synonymes

KAP 1, KAP1, KRAB associated protein 1, KRAB interacting protein 1, KRIP 1, Nuclear corepressor KAP 1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1B, TRIM28, tripartite motif containing 28

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Galerie de données de validation

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  WB analysis of HeLa using 66630-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66630-1-Ig

  WB result of KAP1 antibody (66630-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HepG2 cells.

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  IP experiment of HeLa using 66630-1-Ig

  IP result of anti-KAP1 (IP:66630-1-Ig, 5ug; Detection:66630-1-Ig 1:1000) with HeLa cells lysate 4000 ug.

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  IHC staining of human breast cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66630-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66630-1-Ig (KAP1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66630-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

66630-1-Ig cible KAP1 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KAP1 Protéine recombinante Ag7519
• Nom complet: tripartite motif-containing 28
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC004978
• Symbole du gène: TRIM28
• Identification du gène (NCBI): 10155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. （PMID: 18590578 ）

Publications

Species	Application	Title
human	WB	Sci Adv; Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates.; Authors - Wenli Yang
human	WB	Nucleic Acids Res; SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair.; Authors - Tianyun Hou
human	WB	BMC Pulm Med; miR-125b-5p upregulation by TRIM28 induces cisplatin resistance in non-small cell lung cancer through CREB1 inhibition; Authors - Qiuyu Tan ; KD Validated
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
human	WB	J Mol Biol; Mechanism of SUMOylation-Mediated Regulation of Type I IFN Expression; Authors - Li Du
human	WB,ChIP	Oncogene; ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression; Authors - Lifang Li