Anticorps Polyclonal de lapin anti-JUN

• Phare
• Validé par KD/KO

JUN Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris, Hamster

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-24909

Synonymes

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

---

Galerie de données de validation

• 

  IF Staining of NIH/3T3 using CL488-24909

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 JUN antibody (CL488-24909) at dilution of 1:200, CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en IF: cellules NIH/3T3,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-24909 cible JUN dans les applications de IF et montre une réactivité avec des échantillons Humain, souris, Hamster

• Réactivité: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JUN Protéine recombinante Ag17639
• Nom complet: jun oncogene
• Masse moléculaire calculée: 331 aa, 36 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC068522
• Symbole du gène: JUN
• Identification du gène (NCBI): 3725
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis[PMID: 22180088]. JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription[PMID: 9732876]. In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression[PMID:8464713]. More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins[PMID: 15469925]. This antibody is a rabbit polyclonal antibody raised against a region of human JUN. Both phosphorylated (p-c-Jun) and unphosphorylated forms of c-Jun, with sizes of 42-45 kDa and 36-39 kDa, respectively are obtain in some experiments. (PMID: 17210646)