Anticorps Monoclonal anti-JUN

JUN Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

20

CloneNo.

1G6B11

N° de cat : 66313-1-Ig

Synonymes

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 66313-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66313-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66313-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66313-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66313-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66313-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules A431, cellules Jurkat, cellules NIH/3T3, cellules ROS1728

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below

Informations sur le produit

66313-1-Ig cible JUN dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: JUN Protéine recombinante Ag17639
• Nom complet: jun oncogene
• Masse moléculaire calculée: 331 aa, 36 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC068522
• Symbole du gène: JUN
• Identification du gène (NCBI): 3725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of JUN?

The molecular weight of JUN is approximately 39 kDa.

What is the cellular localization of JUN?

JUN expression can be located in the nucleus. However, ubiquitinated JUN colocalizes with lysosomal proteins (PMID: 15469925).

 What is the function of JUN?

JUN is a basic leucine zipper (bZIP) transcription factor that recognizes and binds to a consensus heptamer motif of 5'-TGA[CG]TCA-3' in the enhancer region of target genes. JUN acts as either a homo- or heterodimer and binds to the DNA to regulate transcriptional activity, specifically promoting the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon the stimulation of the cAMP signaling pathway (PMID: 9732876).

 What post-translational modifications is JUN subjected to?

JUN is acetylated at Lys-271 by EP300 (PMID: 11689449). JUN is phosphorylated by PLK3 following exposure to hypoxia or UV irradiation, which leads to increased DNA-binding activity (PMID: 27281822), and also by PAK2 at numerous threonine residues to promote cell proliferation and transformation (PMID: 21177766). JUN is also phosphorylated by DYRK2 at Ser-243, which primes it for subsequent further phosphorylation by GSK3B and reduces its ability to bind DNA (PMID: 22307329).

 What is the role of JUN in disease?

Activation of JUN is linked with proliferation and angiogenesis in invasive breast cancers (PMID: 16733206).

Publications

Species	Application	Title
mouse	WB	J Hematol Oncol; PMID: 32552902; Authors - Xinhua Xiao
mouse,human	WB	Cell Biol Toxicol; microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.; Authors - Qiuyue Li
human	WB	Genomics; Integrative epigenomic profiling reveal AP-1 is a key regulator in intrahepatich cholangiocarcinoma.; Authors - Ke He
human	WB	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
mouse	WB	J Cell Physiol; Lack of Mof reduces acute liver injury by enhancing transcriptional activation of Igf1.; Authors - Meng Wang
mouse	WB	Front Pharmacol; Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.; Authors - Xiang Tong