Anticorps Polyclonal de lapin anti-Involucrin

Involucrin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 28462-1-AP

Synonymes

involucrin, IVL

---

Galerie de données de validation

• 

  WB analysis of A431 using 28462-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 28462-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 28462-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Involucrin antibody (28462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 28462-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Involucrin (28462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HT-1080, cellules HT-29
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

28462-1-AP cible Involucrin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Involucrin Protéine recombinante Ag29585
• Nom complet: involucrin
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_005547
• Symbole du gène: IVL
• Identification du gène (NCBI): 3713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

Publications

Species	Application	Title
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	IF	Int Immunopharmacol; Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway.; Authors - Jie-Fang Gao
mouse	WB,IHC	J Ethnopharmacol; Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.; Authors - Zhenzhuo Li
human	WB	J Oncol; Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma; Authors - Shiyu Zeng
human	WB	Biol Pharm Bull; Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes; Authors - Yushi Katsuyama
human,mouse	WB	Front Pharmacol; Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation; Authors - Lusheng Wang