Anticorps Monoclonal anti-CD61 / Integrin Beta 3

• Phare
• Validé par KD/KO

CD61 / Integrin Beta 3 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

3D8E5

N° de cat : 66952-1-Ig

Synonymes

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

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Galerie de données de validation

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  WB analysis of HUVEC using 66952-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66952-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66952-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66952-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

66952-1-Ig cible CD61 / Integrin Beta 3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD61 / Integrin Beta 3 Protéine recombinante Ag27834
• Nom complet: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Masse moléculaire calculée: 788 aa, 87 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC127666
• Symbole du gène: ITGB3
• Identification du gène (NCBI): 3690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC	Sci Rep; Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer.; Authors - Hiroyuki Yamato ; KD Validated
human	WB	Reprod Biol Endocrinol; Expression of Talin-1 in endometriosis and its possible role in pathogenesis.; Authors - Xian Tang
human	WB,IP	BMC Biotechnol; αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8.; Authors - Junxin Mai
human	WB	Acta Pharmacol Sin; Adiponectin receptor agonist AdipoRon modulates human and mouse platelet function.; Authors - Xiang-Hui Zhou
mouse	WB	Drug Deliv; Suppression for lung metastasis by depletion of collagen I and lysyl oxidase via losartan assisted with paclitaxel-loaded pH-sensitive liposomes in breast cancer; Authors - Li Zhang
human	WB,IP	Biomedicines; ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis; Authors - Liansuo Zhang