Anticorps Polyclonal de lapin anti-Integrin alpha-1

Integrin alpha-1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 29042-1-AP

Synonymes

CD49a, Integrin alpha 1, integrin, alpha 1, ITGA1, Laminin and collagen receptor, VLA 1, VLA1

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Galerie de données de validation

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  WB analysis using 29042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29042-1-AP cible Integrin alpha-1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha-1 Protéine recombinante Ag30613
• Nom complet: integrin, alpha 1
• Masse moléculaire calculée: 1179 aa, 131 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC137121
• Symbole du gène: ITGA1
• Identification du gène (NCBI): 3672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Publications

Species	Application	Title
human	WB	Development; TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration; Authors - Binbin Li