Anticorps Polyclonal de lapin anti-Involucrin

Involucrin Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 55328-1-AP

Synonymes

involucrin, IVL

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Galerie de données de validation

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  WB analysis of Raji using 55328-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55328-1-AP (IVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55328-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55328-1-AP (IVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human thymus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human oesophagus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55328-1-AP (Involucrin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55328-1-AP (Involucrin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse skin using 55328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using 55328-1-AP (Involucrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu de thymus humain, tissu œsophagien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cutané de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

55328-1-AP cible Involucrin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: involucrin
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 80-90 kDa, 56-70 kDa
• Numéro d’acquisition GenBank: NM_005547
• Symbole du gène: IVL
• Identification du gène (NCBI): 3713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)

Publications

Species	Application	Title
human	IF	Sci Rep; Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.; Authors - Jiuxiang Wang
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	WB	Oncotarget; Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway.; Authors - Sheng Chen
human	WB	Front Oncol; Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program; Authors - Tian-Tian Xu
human	WB	Int J Mol Sci; Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell.; Authors - Chiang-Wen Lee
mouse	IHC	Mar Drugs; Fucoidan from Undaria pinnatifida Ameliorates Epidermal Barrier Disruption via Keratinocyte Differentiation and CaSR Level Regulation.; Authors - Yu Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Involucrin Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:55328-1-AP)