Anticorps Monoclonal anti-Inhibin beta A-Specific

Inhibin beta A-Specific Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

CloneNo.

1F8D8

N° de cat : 60352-1-Ig

Synonymes

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A

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Galerie de données de validation

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  WB analysis of HepG2 using 60352-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 60352-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60352-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 60352-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:650 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, tissu hépatique de porc, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'estomac humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60352-1-Ig cible Inhibin beta A-Specific dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: inhibin, beta A
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: NM_002192
• Symbole du gène: INHBA
• Identification du gène (NCBI): 3624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.